CRISPR/Cas9 mutagenesis of the Arabidopsis GROWTH-REGULATING FACTOR (GRF) gene family
Genome editing in plants typically relies on T-DNA plasmids that are mobilized by Agrobacterium-mediated transformation to deliver the CRISPR/Cas machinery.Here, we introduce olea europaea montra a series of CRISPR/Cas9 T-DNA vectors for minimal settings, such as teaching labs.Gene-specific targeting sequences can be inserted as annealed short olig